ANALYSIS OF CRYPTOCOCCAL EXTRACELLULAR VESICLES: EXPERIMENTAL APPROACHES FOR STUDYING THEIR DIVERSITY AMONG MULTIPLE ISOLATES, KINETICS OF PRODUCTION, METHODS OF SEPARATION, AND DETECTION IN CULTURES OF TITAN CELLS

Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells

ABSTRACT Extracellular vesicles (EVs) produced by members of the Cryptococcus genus are associated with fundamental processes of fungal physiology and virulence.However, several questions about the properties of cryptococcal EVs remain unanswered, mostly because of technical limitations.We recently described a fast and efficient protocol of high-yi

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Study of glycerol diffusion in skin and myocardium ex vivo under the conditions of developing alloxan-induced diabetes

Diabetes mellitus is one of widespread diseases, the development of which impairs the functioning of organism vital organs.The study of the processes that occur in tissues under diabetic conditions facilitates the development of diabetes diagnostics and treatment methods, as well as the prognosis of its complications.The paper reports a comparative

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